期刊
JOURNAL OF HISTOTECHNOLOGY
卷 36, 期 3, 页码 86-91出版社
MANEY PUBLISHING
DOI: 10.1179/014788813X13756994210382
关键词
Ejection; Histotechnology; Laminectomy; Mouse; Rat; Rodent; Rodent necropsy; Spinal cord
类别
For researchers seeking to collect spinal cord samples from mice and rats while avoiding acid decalcification, few options are available. Laminectomy is the standard method which yields high quality samples, yet is time consuming and technically difficult. Ejection of the cord from the vertebral column is another technique commonly used; however, the literature suggests that this method can damage the spinal tissues and is typically avoided when histology of samples is the desired endpoint. Here, we describe an optimized method for ejection of spinal cords from rats and mice, and compare histological quality of these samples with those collected via laminectomy. Our results show that ejection can yield high quality spinal cord samples and may be suitable for use as an alternative to laminectomy.
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