期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 61, 期 6, 页码 407-420出版社
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155413484152
关键词
lipid nanoparticle; LNP; siRNA; biodistribution; QWBA; intravital imaging; stem-loop PCR; RISC; endosomal escape; delivery; immunofluorescence staining
类别
资金
- Merck
Small interfering RNA (siRNA) therapeutics have advanced from bench to clinical trials in recent years, along with new tools developed to enable detection of siRNA delivered at the organ, cell, and subcellular levels. Preclinical models of siRNA delivery have benefitted from methodologies such as stem-loop quantitative polymerase chain reaction, histological in situ immunofluorescent staining, endosomal escape assay, and RNA-induced silencing complex loading assay. These technologies have accelerated the detection and optimization of siRNA platforms to overcome the challenges associated with delivering therapeutic oligonucleotides to the cytosol of specific target cells. This review focuses on the methodologies and their application in the biodistribution of siRNA delivered by lipid nanoparticles.
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