期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 61, 期 5, 页码 382-395出版社
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155413478613
关键词
scanning thin-sheet laser imaging microscopy; light-sheet fluorescence microscopy; orthogonal-plane fluorescence optical sectioning microscopy; micro-computed tomography; mouse cochlea
类别
资金
- Research Foundation-Flanders
- VOCATIO foundation
- NIDCD [RO1DC007588-04]
- ARRA [RO1DC007588-03S1]
- Capita Foundation
- Lions Hearing Foundation
We made a qualitative and quantitative comparison between a state-of-the-art implementation of micro-Computed Tomography (microCT) and the scanning Thin-Sheet Laser Imaging Microscopy (sTSLIM) method, applied to mouse cochleae. Both imaging methods are non-destructive and perform optical sectioning, respectively, with X-rays and laser light. MicroCT can be used on fresh or fixed tissue samples and is primarily designed to image bone rather than soft tissues. It requires complex back-projection algorithms to produce a two-dimensional image, and it is an expensive instrument. sTSLIM requires that a specimen be chemically fixed, decalcified, and cleared; but it produces high-resolution images of soft and bony tissues with minimum image postprocessing and is less expensive than microCT. In this article, we discuss the merits and disadvantages of each method individually and when combined.
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