期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 58, 期 5, 页码 463-479出版社
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.2010.954768
关键词
agrin; intermediate lobe; laminin; neurohypophysis; perlecan
类别
资金
- Hungarian Scientific Research Fund [60930]
The so-called neurointermediate lobe is composed of the intermediate and neural lobes of the pituitary. The present immunohistochemical study investigated components of the basal lamina (laminin, agrin, and perlecan), the dystrophin dystroglycan complex (dystrophin, beta-dystroglycan,alpha 1-dystrobrevin, beta-dystrobrevin, utrophin, and alpha 1-syntrophin), and the aquaporins (aquaporin-4 and -9). Glia markers (GFAP, S100, and glutamine synthetase) and components of connective tissue (collagen type I and fibronectin) were also labeled. In the neurohypophysis, immunostaining of basal lamina delineated meningeal invaginations. In these invaginations, vessels were seen to penetrate the organ without submerging into its parenchyma. On the parenchymal side of the invaginations, beta-dystroglycan was detected, whereas utrophin was detected in the walls of vessels. Immunostaining of alpha 1-dystrobrevin and alpha 1-syntrophin did not delineate the vessels. The cells of the intermediate lobe were fully immunoreactive to alpha 1-dystrobrevin and alpha 1-syntrophin, whereas components of the basal lamina delineated the contours of the cells. GFAP-immunoreactive processes surrounded them. Aquaporin-4 localized at the periphery of the neurohypophysis, mainly adjacent to the intermediate lobe but not along the vessels. It colocalized only partially with GFAP and not at all with alpha 1-syntrophin. Aquaporin-9 was not detected. These results emphasize the possibility that the components of the dystrophin dystroglycan complex localize differently and raise the question about the roles of dystrobrevins, alpha 1-syntrophin, and aquaporin-4 in the functions of the intermediate and neural lobes, respectively. (J Histochem Cytochem 58:463-479, 2010)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据