期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 57, 期 9, 页码 889-897出版社
HISTOCHEMICAL SOC INC
DOI: 10.1369/jhc.2009.953901
关键词
ADAMTS; intimal atrophy; flow; proteoglycan; smooth muscle cells
类别
资金
- National Institutes of Health, U.S. Public Health Service [HL30946, RR00166, HL07828]
- Shriners Hospitals
- Arthritis Foundation
High blood flow through baboon polytetrafluorethylene aorto-iliac grafts increases neointimal vascular smooth muscle cell (SMC) death, neointimal atrophy, and cleavage of versican to generate the DPEAAE neoepitope, a marker of ADAMTS-mediated proteolysis. in this study, we have determined the effect of high blood flow on transcript abundance in the neointima for ADAMTS1, -4, -5, -8, -9, -15, and -20. We found that after 24 hr of flow, the mRNA for ADAMTS4 was significantly increased, whereas that for the other family members was unchanged. Because vascular SMC death is markedly increased in the graft after 24 hr of high flow, we next examined the possibility that the ADAMTS4 induction and the cell death are causally related. The addition of Fas ligand to SMC cultures increased both ADAMTS4 mRNA and cell death similar to 5-fold, consistent with the idea that ADAMTS4-dependent cleavage of versican may be partly responsible for cell death and tissue atrophy under these conditions. (J Histochem Cytochem 57:889-897, 2009)
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