期刊
JOURNAL OF HEREDITY
卷 102, 期 5, 页码 622-626出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/jhered/esr070
关键词
Conservation genetics; microsatellite markers; endangered species; Scleractinia
资金
- BBVA Foundation
- Spanish Ministry of Science and Innovation [CTM2008-00496/MAR]
- National Science Foundation [NSF-OCE-04-24996]
- Directorate For Geosciences
- Division Of Ocean Sciences [926822] Funding Source: National Science Foundation
Cladocora caespitosa is a reef-building zooxanthellate scleractinian coral in the Mediterranean Sea. Mortality events have recurrently affected this species during the last decade. Thus, knowledge of its genetic structure, population diversity, and connectivity is needed to accomplish suitable conservation plans. In order to obtain a better understanding of the population genetics of this species, 13 highly variable microsatellites markers were developed from a naturally bleached colony. The developed primers failed to amplify zooxanthella DNA, isolated from C. caespitosa, verifying that these markers were of the coral and not algal symbiont origin. The degree of polymorphism of these loci was tested on tissue samples from 28 colonies. The allele number for each loci ranged from 2 to 13 (mean N-a = 5.4), with an average observed heterozygosity of 0.42 (H-e = 0.43) and all loci were in Hardy-Weinberg equilibrium. These new markers should be useful in future conservation genetic studies and will help to improve the resolution of the individual identification within this coral species. Primers were also tested in Oculina patagonica, with successful amplifications of several loci.
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