期刊
JOURNAL OF HEPATOLOGY
卷 59, 期 4, 页码 723-730出版社
ELSEVIER
DOI: 10.1016/j.jhep.2013.05.042
关键词
Th-cell depletion; Recent thymic emigrants; Naive Th cells; Peripheral homeostatic proliferation; Bacterial translocation
资金
- Spanish Ministry of Health, Instituto de Salud Carlos III [PS09/00485, PI051871, PI08/1890]
- Fundacion Mutua Madrile [AP100652012]
- Comunidad de Madrid MITIC-CM [S2010/BMD-2502]
- Instituto de Salud Carlos III
- Spanish Ministry of Education [BES-2008-003209]
Background & Aims: Depletion of circulating CD4(+) T-helper (Th) lymphocytes, especially naive Th cells, is common in cirrhosis. Little is known about the pathogenetic mechanisms involved in Th-cell depletion in cirrhosis. We investigated the mechanisms involved in circulating Th-cell lymphopenia in cirrhosis. Methods: Circulating naive and memory Th cells were analyzed by flow cytometry in 60 patients with cirrhosis and 40 sex-and age-matched healthy controls. Thymopoiesis, apoptosis, cell activation, and proliferation were assessed through CD31, annexin-V, HLA-DR and Ki-67 expression, respectively. Lipopolysaccharide (LPS)-binding protein (LBP) and spleen size were measured as indicators of bacterial translocation and splenic pooling, respectively. Results: Compared to controls, patients showed reduced numbers of Th cells involving a greater depletion of the naive than memory Th-cell compartment (2.7-vs. 1.5-fold, respectively). Recent thymic emigrants were diminished (p < 0.01), and each patient had a lower number of CD31(+) naive Th cells than the matched-control. Spontaneous and induced apoptosis (AnnexinV(+)) of Th cells was increased in patients. Activated (HLA-DR+) and proliferating (Ki-67(+)) memory Th cells were increased in patients (p < 0.01), and they directly correlated with plasma LBP (p < 0.05) and negatively with naive Th cells (p < 0.01), respectively. Naive Th cells were inversely correlated (p < 0.01) with their frequencies of apoptosis and of activated memory Th cells, LBP, and spleen size. On multivariate analysis, defective thymic generation of naive Th cells, increased memory Th-cell activation, and splenomegaly were independently associated with Th-cell depletion. Conclusions: Th-cell immunodeficiency in cirrhosis is explained by a universal defect in thymopoiesis exacerbated by splenic pooling and activation-driven cell-death induced by bacterial translocation. (C) 2013 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
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