期刊
JOURNAL OF HEMATOLOGY & ONCOLOGY
卷 7, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1756-8722-7-17
关键词
pten; cebpa; Myeloid cells; Definitive myelopoiesis; Zebrafish
资金
- National Basic Research Program of China [2007CB947003]
- Strategic Priority Research Program of the Chinese Academy of Science [XDA 01010109]
- National Natural Science Foundation of China [81172694, 31322038, 81370232]
- Research Project of Chinese Ministry of Education [213015A]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Qinglan project [JX2161015124]
- Shanghai Pujiang project [12PJ1410200]
Background: Loss of the tumor suppressor phosphatase and tensin homolog (PTEN) is frequently observed in hematopoietic malignancies. Although PTEN has been implicated in maintaining the quiescence of hematopoietic stem cells (HSCs), its role in hematopoiesis during ontogeny remains largely unexplored. Methods: The expression of hematopoietic marker genes was analyzed via whole mount in situ hybridization assay in ptena and ptenb double mutant zebrafish. The embryonic myelopoiesis was characterized by living imaging and whole mount in situ immunofluorescence with confocal microscopy, as well as cell-specific chemical staining for neutrophils and macrophages. Analyses of the involved signaling pathway were carried out by inhibitor treatment and mRNA injection. Results: Pten-deficient zebrafish embryos exhibited a strikingly increased number of myeloid cells, which were further characterized as being immune deficient. In accordance with this finding, the inhibition of phosphoinositide 3-kinase (PI3K) or the mechanistic target of rapamycin (mTOR) corrected the expansive myelopoiesis in the pten-deficient embryos. Further mechanistic studies revealed that the expression of cebpa, a critical transcription factor in myeloid precursor cells, was downregulated in the pten-deficient myeloid cells, whereas the injection of cebpa mRNA markedly ameliorated the dysmyelopoiesis induced by the loss of pten. Conclusions: Our data provide in vivo evidence that definitive myelopoiesis in zebrafish is critically regulated by pten via the elevation of cebpa expression.
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