期刊
JOURNAL OF HEART AND LUNG TRANSPLANTATION
卷 33, 期 4, 页码 438-445出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.healun.2013.12.004
关键词
chronic heart failure; ventricular function; ventricles; ejection fraction; cardiomyocytes; cell therapy
资金
- Arizona Cancer Center Support Grant
- National Institutes of Health [CA023074]
- Department of Veteran Affairs
- William and Dorothy Shaftner Memorial Award Sarver Heart Center
- Warmer Research Foundation
- Hansjorg Wyss Foundation
- Arizona Biomedical Research Commission
- Biomedical Research and Education Foundation of Southern Arizona
BACKGROUND: Varying strategies are currently being evaluated to develop tissue-engineered constructs for the treatment of ischemic heart disease. This study examines an angiogenic and biodegradable cardiac construct seeded with neonatal cardiomyocytes for the treatment of chronic heart failure (CHF). METHODS: We evaluated a neonatal cardiomyocyte (NCM)-seeded 3-dimensional fibroblast construct (3DFC) in vitro for the presence of functional gap junctions and the potential of the NCM-3DFC to restore left ventricular (LV) function in an in vivo rat model of CHF at 3 weeks after permanent left coronary artery ligation. RESULTS: The NCM-3DFC demonstrated extensive cell-to-cell connectivity after dye injection. At 5 days in culture, the patch contracted spontaneously in a rhythmic and directional fashion at 43 +/- 3 beats/min, with a mean displacement of 1.3 +/- 0.3 mm and contraction velocity of 0.8 +/- 0.2 mm/sec. The seeded patch could be electrically paced at nearly physiologic rates (270 +/- 30 beats/min) while maintaining coordinated, directional contractions. Three weeks after implantation, the NCM-3DFC improved LV function by increasing (p < 0.05) ejection fraction 26%, cardiac index 33%, dP/dt(+) 25%, dP/dt(-) 23%, and peak developed pressure 30%, while decreasing (p < 0.05) LV end diastolic pressure 38% and the time constant of relaxation (Tau) 16%. At 18 weeks after implantation, the NCM-3DFC improved LV function by increasing (p < 0.05) ejection fraction 54%, mean arterial pressure 20%, dP/dt(+) 16%, dP/dt(-) 34%, and peak developed pressure 39%. CONCLUSIONS: This study demonstrates that a multicellular, electromechanically organized cardiomyocyte scaffold, constructed in vitro by seeding NCM onto 3DFC, can improve LV function long-term when implanted in rats with CHF. (C) 2014 International Society for Heart and Lung Transplantation. All rights reserved.
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