期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 278, 期 -, 页码 454-463出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2014.06.039
关键词
Biodegradation; Dioxygenase; Fungal co-culture; Ligninolytic enzymes; Petroleum hydrocarbons
资金
- KAKENHI-Japan [23580230]
- Grants-in-Aid for Scientific Research [23580230] Funding Source: KAKEN
The potential of fungal co-culture of the filamentous Pestalotiopsis sp. NG007 with four different basid-iomycetes - Trametes versicolor U97, Pleurotus ostreatus PL1, Cerena sp. F0607, and Polyporus sp. S133 - for accelerating biodegradation of petroleum hydrocarbons (PHCs) was studied using three different physicochemical characteristic PHCs in soil. All the combinations showed a mutual intermingling mycelial interaction on the agar plates. However, only NG007/S133 (50/50) exhibited an optimum growth rate and enzymatic activities that supported the degradation of asphalt in soil. The co-culture also degraded all fractions at even higher concentrations of the different PHCs. In addition, asphaltene, which is a difficult fraction for a single microorganism to degrade, was markedly degraded by the co-culture, which indicated that the simultaneous biodegradation of aliphatic, aromatic, resin, and asphaltene fractions had occurred in the co-culture. An examination of in-vitro degradation by the crude enzymes and the retrieval fungal culture from the soil after the experiment confirmed the accelerated biodegradation due to enhanced enzyme activities in the co-culture. The addition of piperonyl butoxide or AgNO3 inhibited biodegradation by 81-99%, which demonstrated the important role of P450 monooxygenases and/or dioxygenases in the initial degradation of the aliphatic and aromatic fractions in PHCs. (C) 2014 Elsevier B.V. All rights reserved.
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