期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 260, 期 -, 页码 1100-1107出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2013.06.065
关键词
Mixed contaminated soil; Tolerance; Accumulation; Transgenic alfalfa plants; Human CYP2E1; Glutathione S-transferase
资金
- Qingdao City Science and Technology Bureau [12-1-3-45-nsh]
- National Science Foundation of China (NSFC)
Soil contamination is a global environmental problem and many efforts have been made to find efficient remediation methods over the last decade. Moreover, remediation of mixed contaminated soils are more difficult. In the present study, transgenic alfalfa plants pKHCG co-expressing glutathione S-transferase (GST) and human P4502E1 (CYP2E1) genes were used for phytoremediati on of mixed mercury (Hg)-trichloroethylene (TCE) contaminants. Simultaneous expression of GST andCYP2E1 may produce a significant synergistic effect, and leads to improved resistance and accumulation to heavy metal-organic complex contaminants. Based on the tolerance and accumulation assays, pKHCG transgenic plants were more resistant to Hg/ICE complex pollutants and many folds higher in Hg/TCE-accumulation than the non-transgenic control plants in mixed contaminated soil. It is confirmed that GST and CYP2E1 co-expression may be a useful strategy to help achieve mixed heavy metal-organic pollutants phytoremediation. (C) 2013 Elsevier B.V. All rights reserved.
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