期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 213, 期 -, 页码 378-386出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2012.02.010
关键词
Bioremediation; Enrichment; Mixed cultures; Recalcitrant; Textile effluent
资金
- Department of Biotechnology (DBT)
- Ministry of Science and Technology, New Delhi, India
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
Azo dyes an important group of synthetic compounds are recalcitrant xenobiotics. Conventional technologies are unsuccessful to efficiently remove these compounds from contaminated environment. However, consorted metabolic functioning of innate microbial communities is a promising approach for bioremediation of polluted environment. Bacterial mixed cultures SB4 proficient in complete decolorization of azo dye - Reactive Violet 5R was developed through culture enrichment technique. Bacterial community composition based on 16S rRNA gene analysis revealed that mixed cultures SB4 composed of six bacterial strains namely Bacillus sp. V1DMK. Lysinibacillus sp. V3DMK, Bacillus sp. V5DMK, Bacillus sp. V7DMK, Ochrobacterium sp. V10DMK, Bacillus sp. V12DMK. 584 grew well in minimal medium containing low amount of glucose and yeast extract (YE) (1 g/L) and decolorized 200 mg/L of RV5 within 18 h under static condition. Mixed cultures SB4 decolorized wide range of azo dyes and maximum rate of decolorization was observed at 37 degrees C and pH 7.0. Decolorization efficiency was found to be unaltered under high RV5 and salt concentration where 1500 mg/L of RV5 was decolorized in presence of 20 g/L NaCl. We propose the asymmetric cleavage of RV5 and Fourier transformed infrared (FTIR), NMR and gas chromatography-mass spectrometry (GC-MS) confirmed the formation of four intermediatory compounds 1-diazo-2-naphthol, 4-hydroxybenzenesulphonic acid, 2-naphthol and benzenesulphonic acid. (C) 2012 Elsevier B.V. All rights reserved.
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