期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 192, 期 3, 页码 1746-1755出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2011.07.004
关键词
HPTLC; Decolorization; ADMI; Biotransformation; Dye mixture
资金
- Department of Biotechnology, New Delhi, India
Biodegradation of a mixture containing seven commercial textile dyes with different structures and color properties has been investigated by an ecofriendly strain - Brevi bacillus laterosporus MTCC 2298. It showed 87% decolorization in terms of ADMI removal (American Dye Manufacturing Institute) within 24h. The effective decolorization of dye mixture was attained in the presence of metal salt - CaCl2 and nitrogen sources. The induction of oxido-reductive enzymes such as veratryl alcohol oxidase, tyrosinase, NADH-DCIP reductase and azo reductase was found to be responsible for biotransformation of dyes. High performance thin layer chromatography exposed the mechanism of preferential biodegradation of dyes at different time periods. Significant change in the high pressure liquid chromatography and Fourier transform infrared spectroscopy of sample before and after treatment confirmed the biodegradation of dye mixture. Phytotoxicity study revealed the much less toxic nature of the metabolites produced after the degradation of dyes mixture. (C) 2011 Elsevier B.V. All rights reserved.
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