期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 184, 期 1-3, 页码 89-96出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2010.08.009
关键词
Bioremediation; Uranium (VI); Bio-removal mechanism; Bacterial consortia
资金
- Fundacao para a Ciencia e a Tecnologia [SFRH/BD/29677/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/29677/2006] Funding Source: FCT
The mechanism of uranium (VI) removal by two anaerobic bacterial consortia, recovered from an uncontaminated site (consortium A) and other from an uranium mine (consortium U), was investigated. The highest efficiency of U (VI) removal by both consortia (97%) occurred at room temperature and at pH 7.2. Furthermore, it was found that U (VI) removal by consortium A occurred by enzymatic reduction and bioaccumulation, while the enzymatic process was the only mechanism involved in metal removal by consortium U. FTIR analysis suggested that after U (VI) reduction, U (IV) could be bound to carboxyl, phosphate and amide groups of bacterial cells. Phylogenetic analysis of 16S rRNA showed that community A was mainly composed by bacteria closely related to Sporotalea genus and Rhodocyclaceae family, while community U was mainly composed by bacteria related to Clostridium genus and Rhodocyclaceae family. (c) 2010 Elsevier B.V. All rights reserved.
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