期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 162, 期 1, 页码 50-56出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2008.05.040
关键词
Bacteria; Legume; Heavy metal; CopA; Phylogeny
资金
- National Science Foundation of China [30670372, 30630054]
- Fok Ying Tong Education Foundation [101029]
- RFDP [20050712013]
- PCSIRT
A total of 108 strains of bacteria were isolated from root nodules of wild legumes growing in gold mine tailings in northwest of China and were tested for heavy metal resistance. The results showed that the bacterial strain CCNWRS33-2 isolated from Lespedeza cuneata was highly resistant to copper, cadmium, lead and zinc. The strain had a relatively high mean specific growth rate under each heavy metal stress test and exhibited a high degree of bioaccumulation ability. The partial sequence of the copper resistance gene copA was amplified from the strain and a sequence comparison with our Cu-resistant PCR fragment showed a high homology with Cu-resistant genes from other bacteria. Phylogenetic analysis based on the 16S rRNA gene sequence showed that CCNWRS33-2 belongs to the Rhizobium-Agrobacterium branch and it had 98.9% similarity to Agrobactrium tumefaciens LMG196. (C) 2008 Elsevier B.V. All rights reserved.
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