期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 163, 期 2-3, 页码 1199-1206出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2008.07.086
关键词
Picric acid; Rhodococcus sp NJUST16; Biodegradation; Nitroaromatic compounds
资金
- Foundational Research Program of the Civilian Blasting
- Research Innovation Grant for Graduate of Common High School of Jiangsu Province [AD20246]
A picric acid-degrading bacterium, strain NJUST16, was isolated from a soil contaminated by picric acid and identified as a member of Rhodococcus sp. based oil 16S rRNA sequence. The degradation assays Suggested that the strain NJUST16 could utilize picric acid as the sole source of carbon, nitrogen and energy. The isolate grew optimally at 30 degrees C and initial pH 7.0-7.5 in the mineral salts medium supplemented with picric acid. It was basically consistent with degradation of picric acid by the isolate. Addition of nitrogen sources such as yeast extract and peptone accelerated the degradation of picric acid. However, the stimulation was concentration dependent. The degradation was accompanied by release of stoichiometric amount of nitrite and acidification. The degradation of picric acid at relatively high concentrations (>3.93 mM) demonstrated that the degradation was both pH and nitrite dependent. Neutral and slightly basic pH was crucial to achieve high concentrations of picric acid degradation by the NJUST16 strain. (C) 2008 Elsevier B.V. All rights reserved.
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