期刊
OPTICS EXPRESS
卷 23, 期 12, 页码 16383-16406出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.23.016383
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We describe a new technique based on the use of a high-resolution quadri-wave lateral shearing interferometer to perform quantitative linear retardance and birefringence measurements on biological samples. The system combines quantitative phase images with varying polarization excitation to create retardance images. This technique is compatible with living samples and gives information about the local retardance and structure of their anisotropic components. We applied our approach to collagen fibers leading to a birefringence value of (3.4 +/- 0.3) . 10(-3) and to living cells, showing that cytoskeleton can be imaged label-free. (C) 2015 Optical Society of America
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