期刊
OPTICS EXPRESS
卷 23, 期 4, 页码 5327-5334出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.23.005327
关键词
-
类别
资金
- National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health
- National Heart, Lung, and Blood Institute of the National Institutes of Health
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [ZIAHL005106] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [ZIAEB000074] Funding Source: NIH RePORTER
Three-dimensional super-resolution imaging in thick, semi-transparent biological specimens is hindered by light scattering, which increases background and degrades both contrast and optical sectioning. We describe a simple method that mitigates these issues, improving image quality in our recently developed two-photon instant structured illumination microscope without requiring any hardware modifications to the instrument. By exciting the specimen with three laterally-structured, phase-shifted illumination patterns and post-processing the resulting images, we digitally remove both scattered and out-of-focus emissions that would otherwise contaminate our raw data. We demonstrate the improved performance of our approach in biological samples, including pollen grains, primary mouse aortic endothelial cells cultured in a three-dimensional collagen matrix and live tumor-like cell spheroids. (C) 2015 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据