期刊
OPTICS EXPRESS
卷 23, 期 12, 页码 16142-16153出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.23.016142
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资金
- NIH [R21NS082938, R21MH101688, R01DA035680]
- NSF
We have developed a new open-top selective plane illumination microscope (SPIM) compatible with microfluidic devices, multi-well plates, and other sample formats used in conventional inverted microscopy. Its key element is a water prism that compensates for the aberrations introduced when imaging at 45 degrees through a coverglass. We have demonstrated its unique high-content imaging capability by recording Drosophila embryo development in environmentally-controlled microfluidic channels and imaging zebrafish embryos in 96-well plates. We have also shown the imaging of C. elegans and moving Drosophila larvae on coverslips. (C) 2015 Optical Society of America
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