期刊
OPTICS EXPRESS
卷 23, 期 1, 页码 211-223出版社
OPTICAL SOC AMER
DOI: 10.1364/OE.23.000211
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资金
- Gottfried Wilhelm Leibniz Award - Deutsche Forschungsgemeinschaft (DFG)
- German Ministry of Education and Research (BMBF) within the Biophotonik initiative
Stimulated Emission Depletion (STED) nanoscopy enables multi-color fluorescence imaging at the nanometer scale. Its typical single-point scanning implementation can lead to long acquisition times. In order to unleash the full spatiotemporal resolution potential of STED nanoscopy, parallelized scanning is mandatory. Here we present a dual-color STED nanoscope utilizing two orthogonally crossed standing light waves as a fluorescence switch-off pattern, and providing a resolving power down to 30 nm. We demonstrate the imaging capabilities in a biological context for immunostained vimentin fibers in a circular field of view of 20 mu m diameter at 2000-fold parallelization (i.e. 2000 intensity minima). The technical feasibility of massively parallelizing STED without significant compromises in resolution heralds video-rate STED nanoscopy of large fields of view, pending the availability of suitable high-speed detectors. (C) 2015 Optical Society of America
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