期刊
JOURNAL OF GENETICS AND GENOMICS
卷 38, 期 9, 页码 391-402出版社
SCIENCE PRESS
DOI: 10.1016/j.jgg.2011.08.004
关键词
Azaserine; CTP synthase; Cytoophidium; 6-diazo-5-oxo-L-norleucine; Drosophila; HeLa cell; Intracellular compartmentation
资金
- UK Medical Research Council
- International Association for the Exchange of Students for Technical Experience (IAESTE UK, British Council)
- MRC [MC_U137788471] Funding Source: UKRI
- Medical Research Council [MC_U137788471] Funding Source: researchfish
CTP synthase is compartmentalized within a subcellular structure, termed the cytoophidium, in a range of organisms including bacteria, yeast, fruit fly and rat. Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells. Surprisingly, the occurrence of cytoophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-L-norleucine (DON), an inhibitor of glutamine-dependent enzymes including CTP synthase. Experiments in flies confirmed that DON globally promotes cytoophidium assembly. Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia. Moreover, DON facilitates cytoophidium assembly even when CTP synthase level is low. A second glutamine analog azaserine also promotes cytoophidum formation. Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia.
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