期刊
JOURNAL OF GENERAL VIROLOGY
卷 99, 期 10, 页码 1381-1390出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001130
关键词
infectious bronchitis virus; Coronavirus; chicken; accessory genes; accessory proteins; live attenuated virus
资金
- Boehringer Ingelheim, Ingelheim, Germany
Avian coronavirus infectious bronchitis virus (IBV) infects domestic fowl, resulting in respiratory disease and causing serious losses in unprotected birds. Its control is mainly achieved by using live attenuated vaccines. Here we explored the possibilities for rationally attenuating IBV to improve our knowledge regarding the function of IBV accessory proteins and for the development of next-generation vaccines with the recently established reverse genetic system for IBV H52 based on targeted RNA recombination and selection of recombinant viruses in embryonated eggs. To this aim, we selectively removed accessory genes 3a, 3b, 5a and 5b individually, and rescued the resulting recombinant (r) rIBV-D 3a, rIBV-D 3b, rIBV-D 5a and rIBV-D 5b. In vitro inoculation of chicken embryo kidney cells with recombinant and wild-type viruses demonstrated that the accessory protein 5b is involved in the delayed activation of the interferon response of the host after IBV infection. Embryo mortality after the inoculation of 8-day-old embryonated chicken eggs with recombinant and wild-type viruses showed that rIBV-D 3b, rIBV-D 5a and rIBV-D 5b had an attenuated phenotype in ovo, with reduced titres at 6 h p.i. and 12 h p.i. for all viruses, while growing to the same titre as wild-type rIBV at 48 h p.i. When administered to 1-day-old chickens, rIBV-D 3a, rIBV-D 3b, rIBV-D 5a and rIBV-D 5b showed reduced ciliostasis in comparison to the wild-type viruses. In conclusion, individual deletion of accessory genes in IBV H52 resulted in mutant viruses with an attenuated phenotype.
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