期刊
JOURNAL OF GENERAL VIROLOGY
卷 95, 期 -, 页码 2285-2296出版社
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.067710-0
关键词
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资金
- Iowa State University Plant Sciences Institute Virus-Insect Interactions Initiative
- USDA-NRI/AFRI Pest and Beneficial Insects in Plant Systems [2012-67013-19295]
The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genonne sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus lflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 Was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNA. We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.
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