期刊
JOURNAL OF GENERAL VIROLOGY
卷 93, 期 -, 页码 2646-2651出版社
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/vir.0.045146-0
关键词
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资金
- Institut Pasteur the CNRS
- DGA (Direction Generale des Armees)
- Ecole Norma le Superieure de Lyon
- INSERM
- Programme interdisciplinaire CNRS Maladies infectieuses emergentes
- NIH [AI072495-01]
RNA editing mediated by adenosine deaminases acting on RNA (ADARs) converts adenosine (A) to inosine (I) residues in dsRNA templates. While ADAR-1-mediated editing was essentially described for RNA viruses, the present work addresses the issue for two delta-retroviruses, human T-cell leukemia virus type 2 and simian T-cell leukemia virus type 3 (HTLV-2 and STLV-3). We examined whether ADAR-1 could edit HTLV-2 and STLV-3 virus genomes in cell culture and in vivo. Using a highly sensitive PCR-based method, referred to as 3DI-PCR, we showed that ADAR-1 could hypermutate adenosine residues in HTLV-2. STLV-3 hypermutation was obtained without using 3DI-PCR, suggesting a higher mutation frequency for this virus. Detailed analysis of the dinucleotide editing context showed preferences for 5' ArA and 5' UrA. In conclusion, the present observations demonstrate that ADAR-1 massively edits HTLV-2 and STLV-3 retroviruses in vitro, but probably remains a rare phenomenon in vivo.
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