期刊
JOURNAL OF GENERAL VIROLOGY
卷 90, 期 -, 页码 2581-2591出版社
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.012815-0
关键词
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资金
- Centre for Virology
- Hampstead Campus
- Royal Free and University Medical School of UCL
- Biotechnology and Biological Sciences Research Council [EGM17738] Funding Source: researchfish
The lytic gene expression of several members of the human herpesvirus family has been profiled by using gene-expression microarrays; however, the lytic cascade of roseoloviruses has not been studied in similar depth. Based on the complete DNA genome sequences of human herpesvirus 6 variant A (HHV-6A) and variant B (HHV-6B), we constructed a cDNA microarray containing DNA probes to their predicted open reading frames, plus 914 human genes. Gene-expression profiling of HHV-6B strain Z29 in SupT1 cells over a 60 h time-course post-infection, together with kinetic classification of the HHV-6B genes in the presence of either cycloheximide or phosphonoacetic acid, allowed the placement of HHV-6B genes into defined kinetic classes. Eighty-nine HHV-6B genes were divided into four different expression kinetic classes: eight immediate-early, 44 early, 33 late and four biphasic. Clustering of genes with similar expression profiles implied a shared function, thus revealing possible roles of previously uncharacterized HHV-6B genes.
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