期刊
JOURNAL OF GENERAL VIROLOGY
卷 90, 期 -, 页码 1734-1740出版社
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.010355-0
关键词
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资金
- NIAID NIH HHS [R01 AI047446, R01 AI047446-09, R01 AI047446-08] Funding Source: Medline
- Grants-in-Aid for Scientific Research [21380187] Funding Source: KAKEN
The efficient incorporation of influenza virus genome segments into virions is mediated by cis-acting regions at both ends of the viral RNAs. It was shown previously that nt 16-26 at the 3' end of the non-structural (NS) viral RNA of influenza A virus are important for efficient virion incorporation and that nt 27-56 also contribute to this process. To understand further the signalling requirements for genome packaging, this study performed linker-scanning mutagenesis in the latter region and found that nt 27-35 made an appreciable contribution to the efficient incorporation of the NS segment. An NS vRNA library was then generated composed of an RNA population with randomized nucleotides at positions 16-35 such that the virus could select the sequences it required for virion incorporation. The sequences selected differed from the wild-type sequence and no conserved nucleotides were selected. The ability of non-wild-type sequences to function in this manner indicates that the incorporation of influenza A virus genome segments does not absolutely require specific sequences.
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