期刊
JOURNAL OF GENERAL VIROLOGY
卷 89, 期 -, 页码 653-659出版社
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/vir.0.83386-0
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资金
- Medical Research Council [MC_U130184144] Funding Source: Medline
- NHLBI NIH HHS [R01 HL079381, HL079381] Funding Source: Medline
- NIAID NIH HHS [AI47355, R01 AI047355] Funding Source: Medline
- MRC [MC_U130184144] Funding Source: UKRI
- Medical Research Council [MC_U130184144] Funding Source: researchfish
The humoral response to hepatitis C virus (HCV) may contribute to controlling infection. We previously isolated human monoclonal antibodies to conformational epitopes on the HCV E2 glycoprotein. Here, we report on their ability to inhibit infection by retroviral pseudoparticles incorporating a panel of full-length El E2 clones representing the full spectrum of genotypes 1 - 6. We identified one antibody, CBH-5, that was capable of neutralizing every genotype tested. It also potently inhibited chimeric cell culture-infectious HCV, which had genotype 2b envelope proteins in a genotype 2a (JFH-1) background. Analysis using a panel of alanine-substitution mutants of HCV E2 revealed that the epitope of CBH-5 includes amino acid residues that are required for binding of E2 to CD81, a cellular receptor essential for virus entry. This suggests that CBH-5 inhibits HCV infection by competing directly with CD81 for a binding site on E2.
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