期刊
JOURNAL OF GENERAL PLANT PATHOLOGY
卷 80, 期 3, 页码 282-286出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10327-014-0518-2
关键词
Arachis hypogaea; Tospovirus; Frankliniella schultzei
A high incidence of plants with mosaic, chlorotic spots, ringspots, necrosis, smaller leaves, and stunting was observed on peanut crops (Arachis hypogaea L.) in Itapolis, So Paulo State, Brazil. Transmission electron microscope examination of thin sections of infected leaves revealed the presence of spheroidal particles, ca. 80 nm in diameter, suggestive of Tospovirus. A DNA fragment of similar to 600 bp was amplified by RT-PCR from total RNA extracted from infected tissues using primers specific for the nucleocapsid gene of Groundnut ringspot virus (GRSV). Nucleotide and deduced amino acid sequences of the fragments showed high identities with known GRSV isolates.
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