期刊
JOURNAL OF GENERAL PLANT PATHOLOGY
卷 78, 期 2, 页码 127-132出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10327-012-0360-3
关键词
Pea; RNA silencing; Potexvirus
资金
- JSPS/MEXT [20688002, 18108001]
- Grants-in-Aid for Scientific Research [18108001, 20688002] Funding Source: KAKEN
Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS). VIGS is a powerful tool for modifying gene expression in host plants. We constructed a virus vector based on White clover mosaic virus (WC1MV) and demonstrated VIGS of phytoene desaturase (PDS) in pea. Photobleaching of tissues, caused by VIGS of PDS, was observed in restricted areas of upper leaves and stems. We confirmed that the PDS mRNA and subgenomic RNAs of WC1MV were reduced in the photobleached tissues.
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