期刊
JOURNAL OF GENERAL PLANT PATHOLOGY
卷 74, 期 5, 页码 345-354出版社
SPRINGER JAPAN KK
DOI: 10.1007/s10327-008-0117-1
关键词
Arabidopsis; NPR1; PR-1 gene; salicylic acid; SIB A; SigA-binding protein
资金
- National Bio-Resources Project (NBRP) of Ministry of Education, Culture, Sports, Science and Technology, Japan
- New Energy and Industrial Technology Development Organization (NEDO) of Japan
- Program for Promotion of Basic Research Activities for Innovative Biosciences
- Ministry of Education, Science, and Culture of Japan [19580053, 18780028]
- Sumitomo Foundation
- Grants-in-Aid for Scientific Research [18780028, 19580053] Funding Source: KAKEN
SigA-binding protein (SIB A) is a nuclear-encoded chloroplast-targeted protein that interacts with the plastid-encoded plastid RNA polymerase sigma-factor SigA (Sig1). In this study, the SIB A gene responded rapidly to salicylic acid (SA) treatment, but responded slowly to ethylene (ET) and jasmonic acid (JA) treatments as determined with microarray and quantitative real-time RT-PCR analyses. Expression of the SIB A gene increased rapidly, with a peak at 2 h after SA treatment and then decreased gradually. In contrast, expression of the PR-1 gene, a marker gene on the SA defense signaling pathway, increased gradually between 0 and 24 h after SA treatment. In addition, transcription levels of SIB A and PR-1 decreased between 0 and 24 h in the benzothiadiazole (BTH)-treated npr1-1 plants. We suggest that the SIB A gene is downstream of NPR1 on the SA signaling pathway. From the results of our analysis of the 1.5-kbp promoter region of the SIB A gene in Arabidopsis, the isolated promoter region of the SIB A gene seems able to drive the transcription of the GUS gene to elevated levels more rapidly in transgenic Arabidopsis plants undergoing SA treatment compared with levels of PR-1. To analyze biological function of SIB A and investigate SIB A-regulated genes, we generated transgenic Arabidopsis plants in which SIB A was overexpressed. As a result, ROS-related genes, such as glutathione transferase, were up-regulated in plants overexpressing SIB A. However, SIB A-overexpressing plants were not resistant to Pseudomonas syringae pv. tomato DC3000 strain. The SIB A gene has promise as a new tool to analyze the SA-signaling pathway and the role of the chloroplast in plant defense responses.
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