Differential regulation of CaV1.2 channels by cAMP-dependent protein kinase bound to A-kinase anchoring proteins 15 and 79/150

The Ca(V)1.1 and Ca(V)1.2 voltage-gated calcium channels initiate excitation-contraction coupling in skeletal and cardiac myocytes, excitation-transcription coupling in neurons, and many other cellular processes. Up-regulation of their activity by the beta-adrenergic-PKA signaling pathway increases these physiological responses. PKA up-regulation of Ca(V)1.2 activity can be reconstituted in a transfected cell system expressing Ca(V)1.2 Delta 1800 truncated at the in vivo proteolytic processing site, the distal C-terminal domain (DCT; Ca(V)1.2[1801-2122]), the auxiliary alpha 2 delta and beta subunits of Ca(V)1.2 channels, and A-kinase anchoring protein-15 (AKAP15), which binds to a site in the DCT. AKAP79/150 binds to the same site in the DCT as AKAP15. Here we report that AKAP79 is ineffective in supporting up-regulation of Ca(V)1.2 channel activity by PKA, even though it binds to the same site in the DCT and inhibits the up-regulation of Ca(V)1.2 channel activity supported by AKAP15. Mutation of the calcineurin-binding site in AKAP79 (AKAP79 Delta PIX) allows it to support PKA-dependent up-regulation of Ca(V)1.2 channel activity, suggesting that calcineurin bound to AKAP79 rapidly dephosphorylates Ca(V)1.2 channels, thereby preventing their regulation by PKA. Both AKAP15 and AKAP79 Delta PIX exert their regulatory effects on Ca(V)1.2 channels in transfected cells by interaction with the modified leucine zipper motif in the DCT. Our results introduce an unexpected mode of differential regulation by AKAPs, in which binding of different AKAPs at a single site can competitively confer differential regulatory effects on the target protein by their association with different signaling proteins.