期刊
JOURNAL OF GENERAL PHYSIOLOGY
卷 135, 期 2, 页码 81-97出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1085/jgp.200910344
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资金
- National Center for Research Resources [P41RR013186]
- NIH [R01 NS08174, R01 GM83913, T32 GM07108]
- Human Frontier Science Program
G protein-coupled receptors (GPCRs) mediate responses to external stimuli in various cell types. Early events, such as the binding of ligand and G proteins to the receptor, nucleotide exchange (NX), and GTPase activity at the G alpha subunit, are common for many different GPCRs. For G(q)-coupled M-1 muscarinic (acetylcholine) receptors (M(1)Rs), we recently measured time courses of intermediate steps in the signaling cascade using Forster resonance energy transfer (FRET). The expression of FRET probes changes the density of signaling molecules. To provide a full quantitative description of M1R signaling that includes a simulation of kinetics in native (tsA201) cells, we now determine the density of FRET probes and construct a kinetic model of M1R signaling through G(q) to activation of phospholipase C (PLC). Downstream effects on the trace membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) and PIP2-dependent KCNQ2/3 current are considered in our companion paper in this issue (Falkenburger et al. 2010. J. Gen. Physiol. doi:10.1085/jgp.200910345). By calibrating their fluorescence intensity, we found that we selected transfected cells for our experiments with similar to 3,000 fluorescently labeled receptors, G proteins, or PLC molecules per mu m(2) of plasma membrane. Endogenous levels are much lower, 1-40 per mu m(2). Our kinetic model reproduces the time courses and concentration-response relationships measured by FRET and explains observed delays. It predicts affinities and rate constants that align well with literature values. In native tsA201 cells, much of the delay between ligand binding and PLC activation reflects slow binding of G proteins to receptors. With M1R and G beta FRET probes overexpressed, 10% of receptors have G proteins bound at rest, rising to 73% in the presence of agonist. In agreement with previous work, the model suggests that binding of PLC to G alpha(q) greatly speeds up NX and GTPase activity, and that PLC is maintained in the active state by cycles of rapid GTP hydrolysis and NX on G alpha(q) subunits bound to PLC.
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