期刊
JOURNAL OF GENERAL PHYSIOLOGY
卷 133, 期 4, 页码 347-359出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1085/jgp.200810075
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资金
- National Institutes of Health [NS08174, GM08391, T32 GM07108, T32 GM07270]
G protein-coupled receptors initiate signaling cascades. M-1 muscarinic receptor (M1R) activation couples through G alpha(q) to stimulate phospholipase C (PLC), which cleaves phosphatidylinositol 4,5-bisphosphate (PIP2). Depletion of PIP2 closes PIP2-requiring Kv7.2/7.3 potassium channels (M current), thereby increasing neuronal excitability. This modulation of M current is relatively slow (6.4 s to reach within 1/e of the steady-state value). To identify the rate-limiting steps, we investigated the kinetics of each step using pairwise optical interactions likely to represent fluorescence resonance energy transfer for M1R activation, M1R/G(beta) interaction, G alpha(q)/G beta separation, G alpha(q)/PLC interaction, and PIP2 hydrolysis. Electrophysiology was used to monitor channel closure. Time constants for M1R activation (<100 ms) and M1R/G beta interaction (200 ms) are both fast, suggesting that neither of them is rate limiting during muscarinic suppression of M current. G alpha(q)/G beta separation and G alpha(q)/PLC interaction have intermediate 1/e times (2.9 and 1.7 s, respectively), and PIP2 hydrolysis (6.7 s) occurs on the timescale of M current suppression. Overexpression of PLC accelerates the rate of M current suppression threefold (to 2.0 s) to become nearly contemporaneous with G alpha(q)/PLC interaction. Evidently, channel release of PIP2 and closure are rapid, and the availability of active PLC limits the rate of M current suppression.
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