期刊
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
卷 55, 期 4, 页码 283-289出版社
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.55.283
关键词
alkalophilic; bleaching; chlorine; halotolerant; indigo; laccase; gamma-proteobacterium; solvents
资金
- Shreyans Industries Limited
- University Grants Commission
An alkalophilic and halotolerant laccase from gamma-proteobacterium JB catalyzed in high concentrations of organic solvents and various salts. The enzyme retained 80-100% activity in 10% concentration of dimethylsulfoxide (DMSO), ethanol, acetone or methanol; 100, 85 and 50% activity in 20 MM MgCl2, 5.0 mm MnCl2 and 0.1 MM CuCl2; 140, 120 and 110% activity in 5.0 mm MnSO4, 10 MM MgSO4 and 1 mm CaSO4, respectively. Sodium halides inhibited the enzyme in the order: F->Br->I->Cl-. In 0.5 M NaCl, pH 6.0, laccase was similar to 60% active. Decolorization of indigo carmine by laccase at pH 9.0 was not inhibited even in the presence of 0.5 M NaCl. Release of chromophoric, reducing and hydrophobic compounds during biobleaching of straw rich-soda pulp by laccase was not inhibited when the enzyme was applied in the presence of 1 M NaCl at pH 8.0. Laccase retained 50% residual activity even when incubated with 5% calcium hypochlorite for 30 min.
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