期刊
JOURNAL OF GENE MEDICINE
卷 11, 期 11, 页码 1064-1070出版社
WILEY
DOI: 10.1002/jgm.1387
关键词
adjuvant; DNA vaccination; EAE; FK506; prevention; tolerance
资金
- China Agricultural University [2007049]
- Doctoral Program Foundation of Institutions of Higher Education of China [2 00 800 191 026]
- State Training Base for Fundamental Research and Teaching of Biological Science [J0730639]
- State Experiment innovation for Undergraduates [20 070 211]
- State Key Laboratory of Agro-Biotechnology [2008SKLAB05-02]
Background DNA vaccination is a strategy that has been developed primarily to elicit protective immunity against infection and cancer. Methods DNA vaccine was used, in conjunction with an immunosuppressant, to tolerize harmful autoimmunity. Results Immunization of C57BL/6 mice with MOG(35-55), a myelin oligodendrocyte glycoprotein-derived peptide, and FK506 (Tacrolimus) as a tolerogenic adjuvant stimulated regulatory dendritic cells, induced antigen-specific regulatory T cells (Treg), and protected the animals from subsequent induction of experimental autoimmune encephalomyelitis (EAE). After EAE there were fewer lymphocytes, including fewer T helper 17 cells, induction, and more Treg infiltrating the spinal cord in the immunized mice compared to in control mice. Furthermore, at the peak of the EAE manifestation, CD4 T cells in the immunized mice showed decreased expression of interferon-gamma and interleukin (IL)-17, but not IL-4, in treated mice. Conclusions DNA vaccination, when applied with an immunosuppressant as adjuvant, can induce antigen-specific tolerance and prevent autoimmune disease. Copyright (C) 2009 John Wiley & Sons, Ltd.
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