ISOLATION AND CHARACTERIZATION OF BIOLOGICALLY ACTIVE SECONDARY METABOLITES FROM METASEQUOIA GLYPTOSTROBOIDES MIKI EX HU

An increase in foodborne diseases has prompted the search for novel antibacterial agents to control foodborne pathogens. In this study, an abietane type diterpenoid, sugiol was isolated from Metasequoia glyptostroboides using a column chromatographic analysis. The structure of the compound was elucidated by spectroscopic data analysis. The sugiol (50 mu g/disc) showed potential antibacterial effect as diameters of inhibition zones (10.2 +/- 0.3 to 16 +/- 0.4 mm) against foodborne pathogenic bacteria. The minimum inhibitory and minimum bactericidal concentrations values of sugiol against the employed bacteria were found in the range of 62.5 to 250 and 125 to 250 mu g/mL, respectively. Also the compound had strong antibacterial effect on the viable counts of the tested bacteria. Scanning electron microscopic study demonstrated potential detrimental effect of sugiol on the morphology of Listeria monocytogenes ATCC 19166. These findings indicate that sugiol could be a possible candidate for using in food industry to control foodborne pathogens.