期刊
JOURNAL OF FOOD PROTECTION
卷 76, 期 1, 页码 129-132出版社
INT ASSOC FOOD PROTECTION
DOI: 10.4315/0362-028X.JFP-12-304
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资金
- (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria-Fonds europeen de developpement regional) of the Spanish Ministry of Economy and Competitiveness [RTA2008-00080-C02, RTA2011-00098-C02]
Packaged raw foods can represent a potential source of Listeria monocytogenes contamination when opened at home, and listeriosis is associated with the consumption of undercooked raw foods. The aim of this study was to characterize a group of L. monocyto genes strains isolated from 56 packages of raw chicken meat from a single brand in order to determine the diversity of the strains that dominate in a particular food over time, as well as their pathogenic potential. Forty (71%) samples were found to be positive for L. monocytogenes, and three isolates per sample were subjected to PCR molecular serotyping. Subtyping of 45 isolates from different manufacturing dates (n = 40) or different molecular serotype within the same sample (n = 5) identified 11 different L. monocyto genes subtypes as defined by pulsed-field gel electrophoresis and sequencing of virulence genes actA and inlA. Two of the subtypes accounted for 51% of the isolates. About 40% of isolates (three subtypes) were found to potentially present attenuated virulence because of the presence of mutations in the prfA and inlA genes.
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