期刊
JOURNAL OF FOOD PROTECTION
卷 74, 期 11, 页码 1883-1890出版社
INT ASSOC FOOD PROTECTION
DOI: 10.4315/0362-028X.JFP-11-242
关键词
-
资金
- Biotechnology Core Facility Branch, CDC
- Division of Field Sciences (DFS) of the FDA
- DFS Challenge Initiative
Assessing the adulteration of food products and the presence of filth and extraneous materials is one of the measures that the U.S. Food and Drug Administration (FDA) utilizes in implementing regulatory actions of public health importance. To date, 22 common pest species (also known as the Dirty 22 species) have been regarded by this agency as the spreaders of foodborne diseases. We have further categorized the Dirty 22 species into four groups: 1 has four cockroach species, 11 has two ant species, has 12 fly species, and TV has four rodent species. The presence of any Dirty 22 species is also considered an indicator of unsanitary conditions in food processing and storage facilities. In this study, we describe the development of a two-step nested PCR protocol to amplify the small subunit ribosomal gene of group I Dirty 22 species that include four cockroach species: Biotic Ila germanica, Blotto orientalis, Periplaneta americana, and Supella longipalpa, along with the development of a PCR restriction fragment length polymorphism method for rapid detection and differentiation of these violative species. This method will be utilized when the specimen cannot be identified with conventional microscopic taxonomic methods, especially when only small body parts are separated and recovered from food samples for analysis or when these body parts are in a decomposed state. This new PCR restriction fragment length polymorphism will provide correct identification of group I Dirty 22 species; this information can then be used in regulation and prevention of foodbome pathogens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据