期刊
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
卷 24, 期 3, 页码 441-448出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2010.08.002
关键词
Vitamin D; Vitamin D-3; 25-Hydroxyvitamin D-3; 25(OH)D-3; LC-MS/MS; Foodstuffs; Pork; Meat; Fish; Eggs; Poultry; Food analysis; Food composition
In order to generate new data for vitamin D content for the Canadian Nutrient File, a method for the quantification of vitamin D-3 and 25(OH)D-3 in foodstuffs has been modified and improved. Vitamin D-3 was quantified using reverse phase liquid chromatography (LC) with UV-diode array detector (UV-DAD), while 25(OH)D-3 was measured by triple quadrupole mass spectrometry (APCI MS/MS). Quantification was by internal standards (IS) using vitamin D-2 and 25(OH)D-2. A Certified Reference Material (CRM-421 containing vitamin D-3) and a control sample (internally generated reference material of ground pork containing both vitamin D-3 and 25(OH)D-3) were used as validation and quality control tools. Limit of detection for both compounds was 0.04 mu g/100 g. Accuracy for vitamin D in the CRM-421 was 99% (0.142 mg/kg for a target of 0.143, n = 10). Recovery of vitamin D-3 in ground pork was 97% (88% absolute recovery). For 25(OH)D-3, a recovery of 94% (73% absolute recovery) was obtained. Using this method, data for vitamin D-3 and 25(OH)D-3 content in a variety of foods (pork, beef, eggs, poultry, fish, and dinners) have been generated. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
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