期刊
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
卷 21, 期 1, 页码 78-83出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2007.07.001
关键词
sugars; Propolis; bee pollen; ligand exchange chromatography; pulsed amperometric detection
The analysis of sugars in the natural products propolis and bee pollen was carried by high-performance thin layer chromatography (HPTLC), high-performance ligand exchange chromatography with pulsed amperometric detection (HPLEC-PAD) and HPLEC coupled to liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) in the positive ion mode. Eleven samples of propolis were analysed, most of which contained no sugars or only traces of sugars. Similar analysis was performed on five samples of bee pollen which were found to contain glucose, fructose and sucrose. These sugars were quantified by HPLEC-PAD. LC-ESI-MS analysis also indicated small amounts of a trisaccharide in the samples. Calibration curves were linear in the range 0.005-0.5 mg/ml and the LOD for all the sugars was < 0.005 mg/ml. The positive ESI-MS of the sugars extracted from bee pollen yielded mainly Ca2+ adducts of sugars, which afforded good sensitivity for their detection. (C) 2007 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据