期刊
JOURNAL OF FLUORESCENCE
卷 24, 期 4, 页码 1055-1059出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-014-1384-1
关键词
Three-channel; Spectral crosstalk; Real-time fluorescence imaging; Apoptosis; Cytosolic pH
资金
- National Nature Science Foundation of China [11374180, 11174172]
- Doctoral Program Research Fund of the Chinese Ministry of Education [20090002110065]
- Tsinghua University Initiative Scientific Research Program [2010THZ01]
Cytosolic pH changes in single thymocytes in the early stages of apoptosis were examined with spectral-crosstalk-improved three-channel real-time fluorescence imaging. Spectral crosstalk was greatly reduced by this improved image processing method. Compared with normal thymocytes, the improved image processing method showed that the cytosolic pH value of thymocytes undergoing apoptosis was lower. A slight diversity in the intracellular pH values of samples subjected to different drug treatments was also observed. This new image processing method is beneficial to the application of the three-channel real-time fluorescence imaging to biological processes.
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