期刊
JOURNAL OF FLUORESCENCE
卷 23, 期 4, 页码 807-812出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-013-1184-z
关键词
i-motif; Fluorescence; pH; Oligonucleotide probe; Pyrene
资金
- National Science Center, Poland [NN204 220040]
In current studies we use the oligonucleotides based on c-myc sequence: CCC CAC CCT CCC CAC CCT CCC C (cmyc22) and CCC CAC CCT CCC CAC CCT CCC CA (cmyc22A) functionalized by pyrene moieties at both termini. Results of the circular dichroism (CD), UV absorption melting experiments, and steady-state fluorescence measurements of pyrene-modified i-motifs as well as their unlabeled precursors are presented and discussed here. The pyrene labels have a remarkable influence on i-motif stability which was deduced from CD spectra and confirmed by UV melting experiments. Both probes emit fluorescence band of pyrene monomer with intensity decreasing upon pH lowering.
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