期刊
JOURNAL OF FLUORESCENCE
卷 21, 期 3, 页码 1249-1254出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-010-0806-y
关键词
Fluorescence quench; Inner filter effects; Correction method; Outer filter effects
资金
- NSFC [20875055]
- Shanghai Tongji Gao Tingyao Environmental Science & Technology Development Fundation
- Self-innovation foundation for graduate students in Shandong University [yzc09040]
- Ministry of Education of China [708058]
- SRF for ROCS, SEM
- Excellent Young Scientists and Key Science-Technology Project in Shandong Province [2008GG10006012]
To identify and eliminate the inner filter effects (IFEs), prepositive and side cells containing absorbents are fixed beside the fluorescer contained cell. In this way, excitation and emission lights can be quenched by primary and secondary outer filter effects respectively, depending on absorbent concentration and cell length. Herein the quenching of emission fluorescence caused by IFEs can be equally reduced by outer filter effects (OFEs) and the interference of IFEs was eliminated. This approach was experimentally used for identifying the interaction mode and mechanism between BSA and nanoAg. Results showed that the quenching of BSA fluorescence and synchronous fluorescence mainly attributes to IFEs, instead of static/dynamic fluorescent quenching. In view of the above, the elimination of the interference of IFEs by the design of OFEs plays an important part in the precise application of fluorescence detector.
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