期刊
JOURNAL OF FLUORESCENCE
卷 22, 期 1, 页码 151-154出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-011-0941-0
关键词
Green fluorescent protein; Chromophore; Fluorescence; Temperature
资金
- Fundacao para a Ciencia e a Tecnologia [SFRH/BPD/26821/2006]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/26821/2006] Funding Source: FCT
The emission behaviour of Aequorea green fluorescent protein (A-GFP) chromophore, in both neutral (N) and anionic (A) form, was studied in the temperature range from 20 degrees C to 75 degrees C and at pH=7. Excitation wavelengths of 399 nm and 476 nm were applied to probe the N and A forms environment, respectively. Both forms exhibit distinct fluorescence patterns at high temperature values. The emission quenching rate, following a temperature increase, is higher for the chromophore N form as a result of the hydrogen bond network weakening. The chromophore anionic form emission maximum is red shifted, upon temperature increase, due to a charge transfer process occurring after A form excitation.
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