期刊
JOURNAL OF FLUORESCENCE
卷 19, 期 3, 页码 561-565出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-008-0424-0
关键词
Two-photon excitation; Fluorescence Correlation Spectroscopy; Michelson interferometer
In the present work, a Michelson interferometer was combined with a two-photon excitation microscope to perform two-focus Fluorescence Correlation Spectroscopy. This simple and original approach allows us to tune the distance between the two excitation volumes and determine absolute diffusion constants. The technique was validated on different model systems that demonstrate the sensitivity of the approach.
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