期刊
JOURNAL OF FISH DISEASES
卷 37, 期 2, 页码 113-121出版社
WILEY
DOI: 10.1111/jfd.12075
关键词
visceral granulomas; Larimichthys crocea; fatty acid methyl ester analysis; Pseudomonas plecoglossicida; multilocus sequence analysis
资金
- Programmer of Special Fund for Agro-scientific Research in the Public Interest [200903029]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0734]
- K.C.Wong Magna Fund in Ningbo University
An enzootic disease characterized by granulomas in internal organs occurred in cage-farmed large yellow croaker, Larimichthys crocea (Richardson), in April and November 2010, in Ningbo, Zhejiang Province. One bacterial strain, named XSDHY-P, was isolated from the diseased fish and identified by biochemical characterization, fatty acid methyl ester (FAME) analysis and multilocus sequence analysis (MLSA). According to the results obtained from the biochemical tests, FAME analysis and phylogenetic analysis derived from 16S ribosomal RNA, gyrB, oprF, oprI, oprL and rpoD gene sequencing, the bacterial isolate, XSDHY-P, was identified as Pseudomonas plecoglossicida. Moreover, lethal dose, 50% trials were carried out to demonstrate the virulence of XSDHY-P in large yellow croaker when administered at 2.13x10(5)colony-forming units per fish. Visceral granulomas were found in the experimentally infected fish as well as in the naturally infected fish, indicating that P.plecoglossicida is another bacterial pathogen that causes granulomatosis in L.crocea.
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