期刊
JOURNAL OF FISH DISEASES
卷 33, 期 3, 页码 261-266出版社
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1365-2761.2009.01119.x
关键词
bacterial load; fallowing period; Piscirickettsia salmonis
资金
- INTESAL Chile
- Marine Harvest Inc. Chile
- Pontificia Universidad Catolica de Valparaiso, Chile
- INNOVA CORFO - Central Chilean government [PTI-11]
A highly sensitive real-time PCR procedure to detect and quantify the number of Pisciricketsia salmonis units in seawater samples from affected farm sites has been developed. The purpose was to determine a fallowing period that would allow safe restocking of the target farm with new fish. Bacterial load was determined in water samples by comparing the obtained amplification values against a standard curve generated by the amplification of known concentrations of the ITS-ribosomal component of P. salmonis DNA, cloned in a suitable vector. The standard curve was linear over the range of 101-1010 log units. Target samples were taken every 10 days over a 40-day period, at 5 m depth and at the surface. In a highly affected area of southern Chile, the number of bacterial units in farm water decreased to zero at day 50. Therefore, a fallowing period of 50 days post-removal of cages of affected fish appears to be appropriate before restocking. This procedure could be adapted to control disease problems because of other pathogens in fish farm waters.
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