期刊
JOURNAL OF FISH DISEASES
卷 32, 期 10, 页码 873-881出版社
WILEY
DOI: 10.1111/j.1365-2761.2009.01066.x
关键词
bacterial cold-water disease; Flavobacterium psychrophilum; loop-mediated isothermal amplification of DNA; quantification
资金
- Grant-in-Aid for Young Scientists (B), KAKENHI [18780153]
- Grant-in-Aid for Scientific Research (C), KAKENHI [19580222]
- The Global COE program, Japan.
- Japan Society for the Promotion of Science for Young Scientists.
- Grants-in-Aid for Scientific Research [18780153, 19580222] Funding Source: KAKEN
Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout fry syndrome of salmonids. The pathogen has been reported from all regions in the world involved in salmonid aquaculture, but also from natural fresh-water environments. We established a quantitative loop-mediated isothermal amplification of DNA (LAMP) method to estimate quantities of F. psychrophilum. LAMP primers were designed based on the sequence of the DNA topoisomerase IV subunit B gene, parE, of F. psychrophilum. parE LAMP exhibited a high specificity for the parE gene of F. psychrophilum but not for other related species. parE LAMP detected the gene in a wide range of concentrations from 2.0 x 101 to 2.0 x 109 copies/reaction within 70 min and revealed a good correlation between threshold times and gene copy number.
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