A rapid qPCR method for genetic sex identification of Salmo salar and Salmo trutta including simultaneous elucidation ofinterspecies hybrid paternity by high-resolution melt analysis

This study presents an improved duplex quantitative polymerase chain reaction (qPCR) method using the master sex-determining gene sdY as a marker for simultaneous genetic sex identification of salmonids of the Salmo genus and paternity elucidation for Salmo salar x Salmo trutta hybrids. This method will provide a new, simple and economical molecular tool for ecological studies of these species as well as for aquaculture purposes. (C) 2014 The Fisheries Society of the British Isles