期刊
JOURNAL OF FISH BIOLOGY
卷 77, 期 2, 页码 414-424出版社
WILEY
DOI: 10.1111/j.1095-8649.2010.02715.x
关键词
Betta smaragdina var; Betta sp; Mahachai; Betta splendens complex; cytochrome c oxidase subunit 1; DNA barcoding; 16S rRNA
资金
- National Research Council of Thailand (NRCT)
- National Sciences and Engineering Research Council of Canada (NSERC)
- Genome Canada through the Ontario Genomics Institute
Two regions of mitochondrial (mt) DNA, cytochrome c oxidase subunit 1 (COI) and 16S rRNA, were sequenced in nine species of Betta from Thailand and Indonesia. Most species showed little intraspecific COI variation (adjusted mean = 0 center dot 48%) including the putative species Betta sp. Mahachai, but one species (Betta smaragdina) included three lineages showing much greater divergence (7 center dot 03-13 center dot 48%) that probably represent overlooked species. These findings were confirmed by maximum likelihood analysis and Bayesian inference, which revealed well-supported corresponding monophyletic clades. Based on these results and morphological differences, the putative species Betta sp. Mahachai from central Thailand is a species distinct from other members of the B. splendens group and represents a new and hitherto undescribed species. Furthermore, this study also demonstrated the probable existence of two overlooked Betta species found in the Khorat plateau basin, illustrating the utility of mitochondrial genetic markers in the revelation of overlooked diversity.
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